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antibodies against postsynaptic density protein 95  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antibodies against postsynaptic density protein 95
    Antibodies Against Postsynaptic Density Protein 95, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibodies+against+postsynaptic+density+protein+95/PSD95+XP+Rabbit+mAb/pmc11936449-56-18-25
    Average 96 stars, based on 654 article reviews
    antibodies against postsynaptic density protein 95 - by Bioz Stars, 2026-10
    96/100 stars

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    Blocking Assay:

    Article Title: Gfap Mutation and Astrocyte Dysfunction Lead to a Neurodegenerative Profile with Impaired Synaptic Plasticity and Cognitive Deficits in a Rat Model of Alexander Disease
    Article Snippet: After transfer (30 V 15 h, Criterion transfer system, 4°C), membranes were stained with REVERT Total Protein Stain and imaged on an Odyssey imager (LI-COR Biosciences) for quantification and normalization of proteins of interest. .. Membranes were then placed in SEA blocking buffer (Pierce, Thermo Fisher Scientific) before proceeding to incubations with primary antibodies against postsynaptic density protein 95 (PSD95; Cell Signaling 3450) or AMPA glutamate receptor subunit A1 (GluA1; Cell Signaling 13185) diluted 1:1,000 in TBS with 0.05% Tween 20 (TTBS) at 4°C overnight. .. Immunoblots were washed three times in TTBS, incubated with a secondary antibody (1:10,000 IRDye-800CW-conjugated goat anti-rabbit, LI-COR 925-32211) at room temperature for 2 h, washed again with TTBS followed by PBS, and allowed to dry before analysis with an Odyssey imager.



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    Fig. 3 Effects of TP consumption on the function of brain in HFD-fed mice. (A) The immunofluorescent staining of Iba1 and <t>PSD-95</t> in hippocampus. (B) The immunohistochemical staining of Iba1 protein in hippocampus. Quantification results of (C1 and C2) immunofluorescent and (C3) immunohistochemical stainings; (D) Protein levels of PSD-95 and BDNF in hippocampus; (E) Relative mRNA levels of Iba1, PSD-95 and BDNF in hippocampus; (F) Relative mRNA levels of Occludin, ZO-1, and Claudin 5 in hippocampus; (G) Relative mRNA levels of inflammatory parameters; (H) The levels of neurotransmitters, 5-HT and NE in hippocampus (n = 4). Data presented as means ± SEM. Bar values with different letters were significantly different by Tukey’s multiple range test (P < 0.05).
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    Fig. 3 Effects of TP consumption on the function of brain in HFD-fed mice. (A) The immunofluorescent staining of Iba1 and <t>PSD-95</t> in hippocampus. (B) The immunohistochemical staining of Iba1 protein in hippocampus. Quantification results of (C1 and C2) immunofluorescent and (C3) immunohistochemical stainings; (D) Protein levels of PSD-95 and BDNF in hippocampus; (E) Relative mRNA levels of Iba1, PSD-95 and BDNF in hippocampus; (F) Relative mRNA levels of Occludin, ZO-1, and Claudin 5 in hippocampus; (G) Relative mRNA levels of inflammatory parameters; (H) The levels of neurotransmitters, 5-HT and NE in hippocampus (n = 4). Data presented as means ± SEM. Bar values with different letters were significantly different by Tukey’s multiple range test (P < 0.05).
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    Fig. 3 Effects of TP consumption on the function of brain in HFD-fed mice. (A) The immunofluorescent staining of Iba1 and <t>PSD-95</t> in hippocampus. (B) The immunohistochemical staining of Iba1 protein in hippocampus. Quantification results of (C1 and C2) immunofluorescent and (C3) immunohistochemical stainings; (D) Protein levels of PSD-95 and BDNF in hippocampus; (E) Relative mRNA levels of Iba1, PSD-95 and BDNF in hippocampus; (F) Relative mRNA levels of Occludin, ZO-1, and Claudin 5 in hippocampus; (G) Relative mRNA levels of inflammatory parameters; (H) The levels of neurotransmitters, 5-HT and NE in hippocampus (n = 4). Data presented as means ± SEM. Bar values with different letters were significantly different by Tukey’s multiple range test (P < 0.05).
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    Fig. 3 Effects of TP consumption on the function of brain in HFD-fed mice. (A) The immunofluorescent staining of Iba1 and <t>PSD-95</t> in hippocampus. (B) The immunohistochemical staining of Iba1 protein in hippocampus. Quantification results of (C1 and C2) immunofluorescent and (C3) immunohistochemical stainings; (D) Protein levels of PSD-95 and BDNF in hippocampus; (E) Relative mRNA levels of Iba1, PSD-95 and BDNF in hippocampus; (F) Relative mRNA levels of Occludin, ZO-1, and Claudin 5 in hippocampus; (G) Relative mRNA levels of inflammatory parameters; (H) The levels of neurotransmitters, 5-HT and NE in hippocampus (n = 4). Data presented as means ± SEM. Bar values with different letters were significantly different by Tukey’s multiple range test (P < 0.05).
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    Fig. 3 Effects of TP consumption on the function of brain in HFD-fed mice. (A) The immunofluorescent staining of Iba1 and <t>PSD-95</t> in hippocampus. (B) The immunohistochemical staining of Iba1 protein in hippocampus. Quantification results of (C1 and C2) immunofluorescent and (C3) immunohistochemical stainings; (D) Protein levels of PSD-95 and BDNF in hippocampus; (E) Relative mRNA levels of Iba1, PSD-95 and BDNF in hippocampus; (F) Relative mRNA levels of Occludin, ZO-1, and Claudin 5 in hippocampus; (G) Relative mRNA levels of inflammatory parameters; (H) The levels of neurotransmitters, 5-HT and NE in hippocampus (n = 4). Data presented as means ± SEM. Bar values with different letters were significantly different by Tukey’s multiple range test (P < 0.05).
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    Fig. 3 Effects of TP consumption on the function of brain in HFD-fed mice. (A) The immunofluorescent staining of Iba1 and <t>PSD-95</t> in hippocampus. (B) The immunohistochemical staining of Iba1 protein in hippocampus. Quantification results of (C1 and C2) immunofluorescent and (C3) immunohistochemical stainings; (D) Protein levels of PSD-95 and BDNF in hippocampus; (E) Relative mRNA levels of Iba1, PSD-95 and BDNF in hippocampus; (F) Relative mRNA levels of Occludin, ZO-1, and Claudin 5 in hippocampus; (G) Relative mRNA levels of inflammatory parameters; (H) The levels of neurotransmitters, 5-HT and NE in hippocampus (n = 4). Data presented as means ± SEM. Bar values with different letters were significantly different by Tukey’s multiple range test (P < 0.05).
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    Fig. 3 Effects of TP consumption on the function of brain in HFD-fed mice. (A) The immunofluorescent staining of Iba1 and <t>PSD-95</t> in hippocampus. (B) The immunohistochemical staining of Iba1 protein in hippocampus. Quantification results of (C1 and C2) immunofluorescent and (C3) immunohistochemical stainings; (D) Protein levels of PSD-95 and BDNF in hippocampus; (E) Relative mRNA levels of Iba1, PSD-95 and BDNF in hippocampus; (F) Relative mRNA levels of Occludin, ZO-1, and Claudin 5 in hippocampus; (G) Relative mRNA levels of inflammatory parameters; (H) The levels of neurotransmitters, 5-HT and NE in hippocampus (n = 4). Data presented as means ± SEM. Bar values with different letters were significantly different by Tukey’s multiple range test (P < 0.05).
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    Image Search Results


    Fig. 3 Effects of TP consumption on the function of brain in HFD-fed mice. (A) The immunofluorescent staining of Iba1 and PSD-95 in hippocampus. (B) The immunohistochemical staining of Iba1 protein in hippocampus. Quantification results of (C1 and C2) immunofluorescent and (C3) immunohistochemical stainings; (D) Protein levels of PSD-95 and BDNF in hippocampus; (E) Relative mRNA levels of Iba1, PSD-95 and BDNF in hippocampus; (F) Relative mRNA levels of Occludin, ZO-1, and Claudin 5 in hippocampus; (G) Relative mRNA levels of inflammatory parameters; (H) The levels of neurotransmitters, 5-HT and NE in hippocampus (n = 4). Data presented as means ± SEM. Bar values with different letters were significantly different by Tukey’s multiple range test (P < 0.05).

    Journal: Food Science and Human Wellness

    Article Title: Tremella polysaccharide mitigates high fat diet-induced anxiety-like behavior through the microbiota-gut-brain axis

    doi: 10.26599/fshw.2024.9250069

    Figure Lengend Snippet: Fig. 3 Effects of TP consumption on the function of brain in HFD-fed mice. (A) The immunofluorescent staining of Iba1 and PSD-95 in hippocampus. (B) The immunohistochemical staining of Iba1 protein in hippocampus. Quantification results of (C1 and C2) immunofluorescent and (C3) immunohistochemical stainings; (D) Protein levels of PSD-95 and BDNF in hippocampus; (E) Relative mRNA levels of Iba1, PSD-95 and BDNF in hippocampus; (F) Relative mRNA levels of Occludin, ZO-1, and Claudin 5 in hippocampus; (G) Relative mRNA levels of inflammatory parameters; (H) The levels of neurotransmitters, 5-HT and NE in hippocampus (n = 4). Data presented as means ± SEM. Bar values with different letters were significantly different by Tukey’s multiple range test (P < 0.05).

    Article Snippet: The obtained protein samples were loaded and separated in sodium dodecylsulfate-polyacrylamide gel electropheresis and transferred onto PVDF membranes, followed by blocking and incubating with primary antibody against postsynaptic density protein-95 (PSD-95) (1:1 000, Cell Signaling Technology, Boston, USA) and BDNF (1:200, Santa Cruz, Heidelberg, Germany).

    Techniques: Staining, Immunohistochemical staining

    Fig. 8 Effect of FMT on brain and colon in HFD-fed mice. (A) The immunofluorescent staining of Iba1 and PSD-95 in hippocampus; (B) Quantification for the expression of Iba1 and PSD-95 in hippocampus; (C) Relative mRNA levels of Iba1, PSD-95 and BDNF in hippocampus; (D) Relative mRNA levels of inflammatory parameters in hippocampus; (E) The immunofluorescent staining of mucus layer; (F) Quantification of mucus thickness; (G-I) Relative mRNA level of ZO-1, Occludin, and Reg3γ in colon (n = 4). Data presented as means ± SEM. Bar values with different letters were significantly different by Tukey’s multiple range test (P < 0.05).

    Journal: Food Science and Human Wellness

    Article Title: Tremella polysaccharide mitigates high fat diet-induced anxiety-like behavior through the microbiota-gut-brain axis

    doi: 10.26599/fshw.2024.9250069

    Figure Lengend Snippet: Fig. 8 Effect of FMT on brain and colon in HFD-fed mice. (A) The immunofluorescent staining of Iba1 and PSD-95 in hippocampus; (B) Quantification for the expression of Iba1 and PSD-95 in hippocampus; (C) Relative mRNA levels of Iba1, PSD-95 and BDNF in hippocampus; (D) Relative mRNA levels of inflammatory parameters in hippocampus; (E) The immunofluorescent staining of mucus layer; (F) Quantification of mucus thickness; (G-I) Relative mRNA level of ZO-1, Occludin, and Reg3γ in colon (n = 4). Data presented as means ± SEM. Bar values with different letters were significantly different by Tukey’s multiple range test (P < 0.05).

    Article Snippet: The obtained protein samples were loaded and separated in sodium dodecylsulfate-polyacrylamide gel electropheresis and transferred onto PVDF membranes, followed by blocking and incubating with primary antibody against postsynaptic density protein-95 (PSD-95) (1:1 000, Cell Signaling Technology, Boston, USA) and BDNF (1:200, Santa Cruz, Heidelberg, Germany).

    Techniques: Staining, Expressing